Chromatography

Chromatogram ?
                       - detector signal vs retention time or volume

x axis : time or volume
y axis : intensity

Chromatography ?

                         - Chromatography is a separation technique based on the different 

                           interactions of compounds with two phases, a mobile phase and a

                           stationary phase, as the compounds travel through a supporting

                           medium.

                          -Components : mobile phase         ; a solvent that flow through the

                                                                          supporting medium

                                               stationary phase     ; a layer or coating on the 

                                                                          supporting medium that

                                                                          interacts with the analytes

                                               supporting medium ; a solid surface on which the 

                                                                          stationary phase is bound or coated

Separation Process and Chromatogram
for chromatography

Retention 

             - related to the strength of the solute's interaction with the mobile and 

               stationary phases.

             - Capacity factor